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Date: 2020-04-03
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SRPS CEN/TS 15633-3:2013

Status: Објављен
Lang: English
Pages: 33
Published: 31.07.2013
National registry: 69/13
Number of decision:
Edition: 1 ED
Related international standards
This standard is identical to:
CEN/TS 15633-3:2012   CEN/TC 275
Price: 3253 RSD

For online reading


Serbian title

Prehrambeni proizvodi – Detekcija alergena u hrani imunološkim metodama – Deo 3: Kvantitativno određivanje lešnika imunoenzimskim ispitivanjem korišćenjem poliklonalnih antitela i detekcije proteina po Loriju

English title

Foodstuffs - Detection of food allergens by immunological methods - Part 3: Quantitative determination of hazelnut with an enzyme immunoassay using polyclonal antibodies and Lowry protein detection

Serbian scope

Ovom tehničkom specifikacijom se utvrđuje metoda imunoadsorpcionog enzimskog ispitivanja (ELISA) za određivanje koncentracije lešnika u uzorcima hrane.

English scope

This Technical Specification specifies an ELISA-method for the determination of hazelnut concentration in food samples. Spiking experiments using diluted ground hazelnut have been used to validate the method’s use on food matrices such as mixed grain cereals, dark chocolate (45 % cocoa) and ice cream. The range of the method is 0,5 mg to 5,0 mg hazelnut protein per kg of food sample. As hazelnut kernels typically contain between 12 % to 15 % protein [2], [3], this equates to approximately 3,7 mg to 37 mg hazelnut kernel per kg of food sample. The upper limit of the range of quantitation can be extended, if required, by further dilution of sample extracts. The method is commercially available ) and has been validated in-house by the manufacturer. The data is included in A.2. The method has been successfully validated by a collaborative study. The study was organized by the Working Group established by the Federal Office of Consumer Protection and Food Safety (BVL) for the execution of § 64 of the German Food and Feed Code (LFGB) for the determination of hazelnut content in dark chocolate. Thirteen German laboratories participated. The data are included in A.3.

Technical committees

E275 - Food analysis - Horizontal methods


67.050 - General methods of tests and analysis for food products


No information added.

National regulation

No information added.

National action

No information added.


allergens, concentration, determination of content, ELISA, food products, food testing, hazelnuts, health protection, Lowry, verification

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